BioLegend ELISA

BioLegend's ELISA app is your one stop shop for all things ELISA. We've included tutorials on how to use our LEGEND MAX™ and ELISA MAX™ Kits and Sets, as well as an active protocol video, product selector, and much much more...

ELISA (Enzyme-linked immunosorbent assay) is a simple, cost-effect technique performed on serum, plasma, cell supernatant, and other biological fluids to determine the presence of an antigen in a sample.

Sandwich ELISAs utilize multiple-well microtiter plates, coated with capture antibodies, to capture soluble proteins. The bound proteins are then detected with a subsequent detection antibody, which is typically labeled with an enzyme, or biotinylated and then followed with streptavidin-enzyme conjugate. A colorimetric substrate is then added, which results in a color change based on the amount of antigen captured. By using a plate reader and plotting resulting values on a standard curve, precise, quantitative values can be obtained.

BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support.
Our product areas include cell immunophenotyping, cytokines and chemokines, adhesion, cancer research, T regulatory cells, stem cells, innate immunity, cell-cycle analysis, apoptosis, and modification-specific antibodies. All of BioLegend's reagents are supported by superior customer service and come with a quality guarantee. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications including:

Flow cytometry and mass cytometry
ELISA and ELISPOT
Immunoprecipitation and ChIP
Western blotting
Immunofluorescence microscopy
Immunohistochemistry
In vitro or in vivo functional assays.

BioLegend offers the broadest selection of fluorochrome conjugates for multi-color flow cytometry. With an experienced biochemistry team, BioLegend is well positioned to offer a wide range of custom conjugation services to meet our customers' specific needs.